Description
Principle of the Assay: Human HE4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HE4 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: E31-F124) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HE4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HE4 amount of sample captured in plate.